![]() ![]() Protein-DNA docking was performed using the HADDOCK v2.4 server, and non-covalent bonds were computed by DNAproDB server. Two 3D DNA structures were built from 5'GCRTG'3 HRE sequence containing the -964G/A alleles using x3DNA. Mouse HIF2 dimer crystal (Protein Data Bank ID: 4ZPK) was used as template to perform homology modelling of human HIF1 quaternary structure using MODELLER v9.14. ![]() ![]() Since the G allele of the -964G > A transversion induces higher HLA-G expression when compared to the A allele in hypoxic conditions, here we analyzed HIF1-HRE complex interaction at the pair-atom level considering both -964G > A polymorphism alleles. Hypoxia regulatory elements (HRE) located at the HLA-G promoter region and at exon 2 are the major HIF1 target sites. To set up the final shot in After Effects, I also cover working with After Effects camera properties and settings, as well as final render settings for Element 3D Version 2.īe sure to check out my previous After Effects Element 3D V2 Tutorial, on my Top 5 features, review, and overall thoughts on E3D Version 2:Įnjoy this free Element 3D After Effects plug-in tutorial and subscribe to my channel for more videos added on a regular basis!ĭid you like this free free Element 3D V2 After Effects plug-in tutorial? Subscribe to my channel for more videos added on a regular basis!ĭon't forget to check out SJanimation.Human Antigen Leukocyte-G ( HLA-G) gene encodes an immune checkpoint molecule that has restricted tissue expression in physiological conditions however, the gene may be induced in hypoxic conditions by the interaction with the hypoxia inducible factor-1 (HIF1). ![]() Version 2 of Element 3D for After Effects by Video Copilot can import Cinema 4D models and animation directly, and I go over this process as well as expand on the possibilities by using a particle replicator and animation engine. In this Element 3D V2 Tutorial, I cover Cinema 4D integration in detail. ![]()
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